Composite

Part:BBa_K3802000:Design

Designed by: Nabeel Farrukh   Group: iGEM21_Stony_Brook   (2021-09-30)


PgsA-MlrA fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 827
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 813
    Illegal NgoMIV site found at 2323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 304
    Illegal BsaI.rc site found at 2160


Design Notes

PgsA is not orginally from E.coli, but rather from Bacillus subtilis. Therefore, we had to codon optimize the entire part to be compatible with E.coli.

Source

This part is a composite of multiple parts from the registry. It was synthesized by a gene synthesizing company (IDT).